Isolated CD34⁺ cells revealed hematopoietic progenitor potential. (A) Schematic diagram of the protocol used for CD34⁺ cells' isolation and analysis. (B) Relative expression of hematopoiesis-associated genes in CD34⁺ and CD34^– populations by QPCR. (C) Clonogenic potential of H1 CD34⁺ and CD34^– cells. Results are mean ± SD of 4 experiments. (D) Rho efflux and CD45 expression by isolated CD34⁺ cells were analyzed by flow cytometry. The gates used to distinguish Rho^low population of CD34⁺CD45⁺ cells and percentages of Rho^low cells within this gate (mean ± SD of 3 experiments; H1 = 2, H9 = 1) are indicated. (E) ALDH and CD45 expression by isolated CD34⁺ cells were analyzed by flow cytometry. The gates used to distinguish the ALDH^high population of CD34⁺CD45⁺ cells and percentages of ALDH^high cells within the gates (mean ± SD of 3 experiments; H1 = 2, H9 = 1) are indicated.