Reduced expression of the FucTVII gene and reduced fucosyltransferase activity in SL^neg cells. Cultured CB-CD34⁺ cells were sorted on the basis of P-selectin binding and sLex expression. (A) RNA was extracted for RT-PCR analyses. The bar graph shows the ratio ± SEM of mRNA levels in SL^neg/SL^pos for each of the genes indicated. Data were normalized for β-actin expression. *P = .003 compared with the β-actin control; n = 4 independent experiments. (B) Cell lysates were assayed for FucT activity. Controls were performed in the absence of a fucose donor (GDP-fucose; hatched bars) for each cell population. Bars represent mean ± SEM. *P = .001, n = 4 independent experiments.