Figure 6.
Treatment with recombinant human fucosyltransferase VI enhances P- and E-selectin binding activity on CB-CD34+CD38lo/neg cells. CB-MNCs were treated with rhFucTVI for 40 minutes at 37°C in the presence of GDP-fucose or left untreated, stained for CD34 and CD38 expression and binding of P- or E-selectin. Shown is CD38 expression and selectin binding of gated CD34+ cells. Numbers on each panel indicate mean ± SEM percentage of CD34+CD38lo/neg cells that bind the selectins. *P = .02, **P = .07, compared with untreated samples; n = 5 donors.