Figure 1.
Two-phase liquid erythroid culture demonstrates a consistent and profound defect in DBA. Total number of hemoglobinized (DAF+) cells (mean ± SEM) generated at different time points in phase 2 of culture, expressed per 1 × 105 total nonadherent cells plated in phase 2. (A) 5637-CM in both phases plus 2 U/mL Epo in phase 2 (normal n = 18; DBA n = 19). (B) Serum-containing medium with IL-3/SCF in both phases plus 2 U/mL Epo in phase 2 (normal, n = 9; DBA, n = 7). (C) Results for 3 first-degree relatives with isolated raised eADA activity, under the same culture conditions as for panel B.
