Analysis of myeloid and lymphoid progenitor cells in Hmgb3^–/Y mice. (A) Mean BFU-Es and CFU-Es per 2 × 10⁴ bone marrow cells isolated from wild-type (n = 8; 135 BFU-Es and 1538 CFU-Es counted) and Hmgb3^–/Y (n = 8; 144 BFU-Es and 2452 CFU-Es counted) mice. To generate BFU-Es and CFU-Es, 2 × 10⁴ and 2 × 10⁵ bone marrow cells were cultured per sample, respectively. (B) Mean CFU-GEMMs and CFU-GMs per 2 × 10⁴ cultured bone marrow cells isolated from wild-type (n = 8; 33 CFU-GEMMs and 190 CFU-GMs counted) and Hmgb3^–/Y (n = 8; 40 CFU-GEMMs and 249 CFU-GMs counted) mice. (C) Mean CFU–Pre-Bs per 5 × 10⁴ cultured bone marrow cells isolated from wild-type (n = 8; 302 CFU–Pre-Bs counted) and Hmgb3^–/Y (n = 8; 449 CFU–Pre-Bs counted) mice. For panels A, B, and C, P values were determined by Student t test. The data represent the pooled results of 2 independent experiments. (D) Average C_t values for TRECs and α-globin real-time PCR performed on CD3⁺ thymocytes harvested from 6-week-old (n = 3) and 1-year-old (n = 3) wild-type and Hmgb3^–/Y mice. C_t represents the number of amplification cycles at which the fluorescent signal in a real-time PCR reaction passes a fixed threshold. For all reactions, C_t values were within the linear range of amplification. Reactions were performed in triplicate for each sample. Error bars in all panels represent standard deviations.