Increased levels of Gata-2 and c-myb in Hmgb3–/Y HSCs. (A) Semiquantitative duplex RT-PCR analysis of selected genes in wild-type and Hmgb3–/Y HSCs (lin–, c-kitHI, Sca-1HI, IL-7Rα–). Test gene mRNA levels were quantified within the linear range of amplification by densitometry and normalized to β2-microglobulin expression. (B) Test gene mRNA levels relative to β2-microglobulin. The amount of test gene mRNA in wild-type (n = 3) and Hmgb3–/Y (n = 3) HSCs was normalized to β2-microglobulin mRNA (β2) by the formula (densitometry value: test/densitometry value: β2-microglobulin). The data represent the pooled results of 3 independent experiments using mRNA isolated from sorted HSCs pooled from 3 mice. Standard deviation values between independent RT-PCR reactions amplifying CCR3 and Gata-1 mRNA from wild-type HSC RNA are not visible on this scale. Error bars represent standard deviation. P values were determined by Student t test.
