Figure 3.
Figure 3. Ectopic expression of hsp70 cDNA in the reverse orientation depletes hsp70 levels and sensitizes CML-BC cells to Ara-C or etoposide-induced apoptosis. (A) Western analyses of the expression of hsp70, hsp90, Bcl-xL, Bcl-2, and XIAP in cells with high (K562, HL-60/hsp70) or low or depleted expression of hsp70 (HL-60, K562/hsp70 AS). (B, C) HL-60/Neo, HL-60/hsp70, K562, and K562/hsp70AS cell were treated with the indicated levels of Ara-C (B) or etoposide (C) for 24 hours. Following these treatments, the percentages of apoptotic cells were determined by annexin-V staining and flow cytometry. Values represent the mean ± SE of 3 experiments.

Ectopic expression of hsp70 cDNA in the reverse orientation depletes hsp70 levels and sensitizes CML-BC cells to Ara-C or etoposide-induced apoptosis. (A) Western analyses of the expression of hsp70, hsp90, Bcl-xL, Bcl-2, and XIAP in cells with high (K562, HL-60/hsp70) or low or depleted expression of hsp70 (HL-60, K562/hsp70 AS). (B, C) HL-60/Neo, HL-60/hsp70, K562, and K562/hsp70AS cell were treated with the indicated levels of Ara-C (B) or etoposide (C) for 24 hours. Following these treatments, the percentages of apoptotic cells were determined by annexin-V staining and flow cytometry. Values represent the mean ± SE of 3 experiments.

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