IL-10-dependent LPS-induced STAT3 activation in WT but not IL-10^-/- BMDCs. BMDCs from WT and IL-10^-/- mice were stimulated with LPS (5 μg/mL) in the presence or absence of IL-10 receptor antibody (30 μg/mL) or an isotype control Ab (30 μg/mL). Subsequently, cells were harvested at various time points and Western blot analysis was performed for (A) phospho-STAT3 and STAT3, (B) phospho-STAT3 and STAT 3 with or without IL-10 receptor blockade. The results are representative of 3 independent experiments.