Angiostatin reduces activity of NFκB and tissue factor expression. The DNA binding activity of NFκB as assessed by EMSA (A) as well as the expression of tissue factor as assessed by Western blot (B) are shown in the absence (–; open bar) or presence of TNFα (▪) without or together with K1-4 (500 nM) or K1-4 + mAb to Mac-1 (10 μg/mL), as indicated. The inserts demonstrate a typical EMSA for NFκB DNA binding activity (A) and a typical blot with staining for tissue factor (B). Densitometric data are presented as percentage of control (in the absence of TNFα or competitor) and are mean ± SD of 3 separate experiments, each performed in triplicate. #P < .05; ns indicates not significant, compared with TNFα alone.