Effect of AS602868 on mitochondrial membrane depolarization and caspase activation in AML blasts. (A) Leukemic blasts from patients 7 and 11 were incubated with indicated doses of AS602868 for 48 hours. Mitochondrial membrane depolarization was assessed by loss of DiOC6(3) staining by flow cytometry. (B) AS602868 induced caspase-3 activation. AML blasts from patients 4, 7, and 11 were incubated with indicated doses of AS602868 for 48 hours before permeabilization and labeling with an antibody against active caspase-3 and analyzed by flow cytometry. (C) AS602868 induces cleavage of PARP. Blasts from patients 4, 7, and 11 were treated with AS602868 (10 μM) for indicated times before analysis of PARP cleavage by Western blotting. Hsp60 levels represent loading controls.