Activated pDCs transcribe the IL-2 gene. (A) Real-time PCR on pDCs after 2, 4, 6, and 16 hours of treatment with CpG, CD40L, or influenza virus. (B) Real-time PCR on pDCs after 4-hour pretreatment with CpG or influenza virus and 12-hour treatment with CD40L. (C) Real-time PCR on activated T cells with anti-CD3 and PMA. (A-C) IL-2 transcripts and 18S rRNA were quantified with real-time florigenic RT-PCR. Transcript abundance is represented as the ratio of IL-2 to 18S rRNA. Error bars represent the standard deviation of 3 different experiments. (D) CD3 profile of the sorted population.