Correlation of TCR-γ PCR results in skin and dermatopathic and effaced lymph nodes. (A-C) Resembles skin and lymph node results of patient no. 7 and (D-F) of patient no. 12, respectively (A,D: skin; B,E: dermatopathic lymph node; C,F: effaced lymph node). Panels A and D show the typical MF histology with epidermotropic atypical lymphocytes present in the skin (with additional Pautrier abscess) in conjunction with the corresponding clonal T-cell populations as demonstrated by TCR-γ PCR and Genescan analysis. The same clonal T-cell populations were also found in the dermatopathic (B and E, → macrophages containing melanin) and effaced lymph node (C, F). Panels A to C (no. 7) revealed an identical peak size of 217 bp, and panels D to F (no. 12) revealed an identical peak size of 195 bp. A,D: × 200. B,C,E,F: × 100.