Expression of the TSLC1 gene in T-cell lines detected by Northern hybridization. Poly(A)⁺ RNA was prepared from various T-cell lines with or without HTLV-1 infection and from ATL cell lines, and TSLC1 expression was analyzed by Northern hybridization. Two bands, 4.4 and 1.6 kb, for TSLC1 transcripts were detected in 2 of 3 T-cell lines with HTLV-1 infection (lanes 4 and 5) and 5 of 7 ATL cell lines (lanes 7, 9, 10, 11, and 12). β-actin expression was used as a control for mRNA quantity.