Methylation analysis of TSLC1. (A) Methylation state of the TSLC1 promoter in 6 CpG sites in 7 ATL, 3 HTLV-1–infected T-cell, and 2 T-ALL cell lines determined by bisulfite-SSCP and sequencing. (B) Methylation state of the TSLC1 promoter in 6 CpG sites in normal CD4⁺ T cells from 10 healthy volunteers and 4 patients with ATL determined by bisulfite-SSCP and sequencing. Black, gray, and white circles represent methylated, partially methylated, and unmethylated CpGs, respectively. Columns correspond to the 6 CpG sites just upstream of the predicted TATA box sequence.