CD11c-Rac1(N17) mice have normal DC numbers but reduced frequencies of CD8⁺ splenic DCs. Splenocytes from Tg⁺ mice and WT controls were prepared as described in “Materials and methods” and stained with mAb specific for CD11c and CD8α as well as PI to exclude dead cells. (A) Representative dot plots are gated on live (PI^-) cells, with staining for CD11c and CD8 as indicated. Histograms are gated on CD11c^hi cells. Cell frequencies within the specified gates are shown. (B) Mean (± SD) values for total splenic cell numbers, DC frequencies, and CD8⁺ DC percentages are shown (n = 7, representative of more than 5 experiments with similar results). Analyses from founder lines F4038 (n = 7) and F4904 (n = 3) are shown. Populations were defined as indicated in panel A.