DC in CD11c-Rac1(N17) mice exhibit no maturation deficiency when stimulated in vivo. Splenic DCs from naive (upper histograms) or LPS/anti-CD40–injected (4 hours after injection, lower histograms) Tg⁺ mice (black lines) and WT controls (gray lines) were stained with mAb specific for CD11c, CD8α, and MHC class II (I-A^b) or CD86. The filled histograms represent naive controls analyzed in parallel with LPS/anti-CD40–injected animals. Histograms are gated on live CD11c⁺ cells, with further separation based on expression of CD8 (CD8⁺ DCs, top panels; CD8^- DCs, bottom panels).