Figure 3.
Figure 3. IFN-α inhibits bone marrow BFU-Es and CFU-Es in a STAT1-independent manner. In vitro hematopoietic colony formation was examined in methylcellulose supplemented with 3% kit ligand-conditioned medium and varying concentrations of IFN-α using bone marrow (A) or splenic cells (B) from wild-type and STAT1–/– mice at 8 to 12 weeks of age. (Ai,Bi) BFU-Es were enumerated 7 days after plating. (Aii,Bii) CFU-Es were counted 2 days after initiation of the experiment. IFN-α concentration/response curve of the growth of (Aiii,Biii) BFU-Es derived and (Aiv,Biv) CFU-E–derived colonies in cultures from wild-type (▪) and STAT1–/– (□) mice. The results are presented as a percentage of the maximal colony growth. Inhibition of BFU-Es and CFU-Es by IFN-γ is dependent on STAT1. In vitro hematopoietic colony formation was examined in methylcellulose supplemented with 3% kit ligand–conditioned medium and varying concentrations of IFN-γ using bone marrow (C) or splenic cells (D) from wild-type and STAT1–/– mice at 8-12 weeks of age. (Ci,Di) BFU-Es were enumerated 7 days after plating. (Cii,Dii) CFU-Es were counted 2 days after initiation of the experiment. IFN-γ concentration/response curve of the growth of (Ciii,Diii) BFU-E–derived and (Civ,Div) CFU-E–derived colonies in cultures from wild-type (▪) and STAT1–/– (□) mice. The results are presented as a percentage of the maximal colony growth.

IFN-α inhibits bone marrow BFU-Es and CFU-Es in a STAT1-independent manner. In vitro hematopoietic colony formation was examined in methylcellulose supplemented with 3% kit ligand-conditioned medium and varying concentrations of IFN-α using bone marrow (A) or splenic cells (B) from wild-type and STAT1–/– mice at 8 to 12 weeks of age. (Ai,Bi) BFU-Es were enumerated 7 days after plating. (Aii,Bii) CFU-Es were counted 2 days after initiation of the experiment. IFN-α concentration/response curve of the growth of (Aiii,Biii) BFU-Es derived and (Aiv,Biv) CFU-E–derived colonies in cultures from wild-type (▪) and STAT1–/– (□) mice. The results are presented as a percentage of the maximal colony growth. Inhibition of BFU-Es and CFU-Es by IFN-γ is dependent on STAT1. In vitro hematopoietic colony formation was examined in methylcellulose supplemented with 3% kit ligand–conditioned medium and varying concentrations of IFN-γ using bone marrow (C) or splenic cells (D) from wild-type and STAT1–/– mice at 8-12 weeks of age. (Ci,Di) BFU-Es were enumerated 7 days after plating. (Cii,Dii) CFU-Es were counted 2 days after initiation of the experiment. IFN-γ concentration/response curve of the growth of (Ciii,Diii) BFU-E–derived and (Civ,Div) CFU-E–derived colonies in cultures from wild-type (▪) and STAT1–/– (□) mice. The results are presented as a percentage of the maximal colony growth.

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