IFN-γ production and STAT4 activation of PECs and BMMΦs of RAG2^-/-γ_c^-/- mice. (A) Total of RAG2^-/-γ_c^-/- PECs or BMMΦs were stimulated with IL-12/IL-18 and intracellular IFN-γ was detected by ICS. Representative for 3 experiments. (B) Production of IFN-γ and TNF of RAG2^-/-γ_c^-/- PECs or BMMΦs after stimulation with IL-12 (with or without IL-18), IL-18, LPS (1μg/mL), or IFN-γ (20 ng/mL) plus LPS (200 ng/mL) for 72 hours. Mean ± SEM of 5 experiments. (C) Analysis of STAT4 activation by EMSA. Nuclear extracts were prepared from PECs or BMMΦs of RAG2^-/-γ_c^-/- mice or from unsorted BALB/c BMMΦs as positive control. The cells remained unstimulated or were stimulated with IL-12/IL-18 for 2 hours (Figure 5). Representative for 3 experiments.