Characterization of functional effects of E26A-RANTES. Surface binding of PF4 was analyzed by flow cytometry in monocytic MM6 cells pretreated with PF4 alone or in combination with E26A-RANTES (A). MM6 cells stimulated with E26A-RANTES alone or in combination with PF4 (200 ng/mL each) were perfused on activated HUVECs. The number of firmly adherent monocytes was determined after accumulation for 5 minutes (B). MM6 cells were preincubated with chondroitinase ABC (0.5 U/mL) for one hour (empty bars). Data represent mean ± SEM of 4 independent experiments (*P < .05 or not significant as indicated).