CD56⁺ cells are involved in FasL-mediated granulocyte migration. (A) Granulocyte migration was evaluated using fMLP (▪) or cross-linked FasL (□). Granulocytes were isolated by Dextran-Ficoll (> 90% pure), CD15⁺ selection (> 98% pure), or MACS depletion with CD3, CD14, CD19, and CD56 microbeads (> 95% pure). (B) Ability of other leukocytes to induce indirect FasL-mediated granulocyte migration. The horizontal line at migration index 1.0 indicates lack of chemotaxis. Cell purity was as follows: CD3 (> 98%), CD14 (> 75%), CD15 (> 98%), CD19 (> 95%), CD56 (> 95%). Data shown are the mean ± SEM of 3 different donors.