T cells expressing CD56 mediate indirect granulocyte chemotaxis. (A) CD3 staining of CD56⁺ cells (> 98% pure) obtained by MACS-positive selection; isotypic control (▩), CD56⁺ cells (□). The profile of 3 different donors is shown. (B) Coreceptor expression pattern in CD56⁺CD3⁺ cells obtained by MACS. (C) Ability of different cell populations to induce indirect FasL-mediated granulocyte migration. The horizontal line at migration index 1.0 indicates lack of chemotaxis. Cell purity was as follows: CD56⁺CD3^- (> 80%), CD56⁺CD3⁺ (> 95%), CD56⁺CD3⁺CD8^- (> 85%), CD56⁺CD3⁺CD8⁺ (> 90%), Vα24⁺ cells (> 90%). Data shown are the mean ± SEM of 3 different donors.