Binding profiles for THC. CEM (•), HEL-92 (HEL; ▪), and HL60 (▴) cell lines were cultured with biotinylated THC. Cells were then incubated with FITC-conjugated streptavidin, and the extent of fluorescence was assessed by flow cytometry. The maximum specific binding (B_max) and the dissociation constant (K_d) values in the cell lines are shown in the inset box. The IgG1 isotype control (control) is also shown, and each data point represents the means and SDs of at least 3 separate experiments.