Figure 7.
Figure 7. Relationship between mutated fractions of VκA27 transcripts and the presence of CD19+/CD27+ memory B cells. (A) Samples from 18 CVID patients were analyzed by VκA27 REHMA and flow cytometry. The abscissa shows the fractions of CD27+ cells among B cells. In 2 patients, the values of mutated fraction and CD27+ fraction are both 0%. (B) CD27 positivity of CD19-gated B cells from one of the 2 CVID patients from whom CD19+/CD27+ memory B cells were sorted. Percentage of B cells (CD19+) negative or positive for CD27 are given in the upper quadrants. (C) Mutated fractions of purified CD19+/CD27+ B cells from 2 CVID patients and 2 healthy adults illustrating reduced light-chain mutation in the patients.

Relationship between mutated fractions of VκA27 transcripts and the presence of CD19+/CD27+ memory B cells. (A) Samples from 18 CVID patients were analyzed by VκA27 REHMA and flow cytometry. The abscissa shows the fractions of CD27+ cells among B cells. In 2 patients, the values of mutated fraction and CD27+ fraction are both 0%. (B) CD27 positivity of CD19-gated B cells from one of the 2 CVID patients from whom CD19+/CD27+ memory B cells were sorted. Percentage of B cells (CD19+) negative or positive for CD27 are given in the upper quadrants. (C) Mutated fractions of purified CD19+/CD27+ B cells from 2 CVID patients and 2 healthy adults illustrating reduced light-chain mutation in the patients.

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