Figure 6.
Figure 6. RNA interference of 3BP2 decreases BCR signaling to NFAT. Jurkat (A) or Raji (B) cells were transfected with 3BP2 siRNA (si3BP2) or control siRNA (siGFP) in the presence of fluorescein-conjugated control siRNA. After 24 hours, cells were sorted by FACS, lysed, and protein expression was determined by immunoblotting with antibodies against 3BP2 and ERK2 (right panels). For determination of NFAT activity in 3BP2 knock-down cells, Jurkat (A) or Raji (B) cells were electroporated with 2 μg siRNA, 15 μg NFAT reporter, and 3 μg Renilla luciferase plasmid. After 18 hours, cells were stimulated or not for 6 hours and luciferase activity was measured and normalized (left panels). Values represent the mean plus or minus the standard deviation from triplicate samples.

RNA interference of 3BP2 decreases BCR signaling to NFAT. Jurkat (A) or Raji (B) cells were transfected with 3BP2 siRNA (si3BP2) or control siRNA (siGFP) in the presence of fluorescein-conjugated control siRNA. After 24 hours, cells were sorted by FACS, lysed, and protein expression was determined by immunoblotting with antibodies against 3BP2 and ERK2 (right panels). For determination of NFAT activity in 3BP2 knock-down cells, Jurkat (A) or Raji (B) cells were electroporated with 2 μg siRNA, 15 μg NFAT reporter, and 3 μg Renilla luciferase plasmid. After 18 hours, cells were stimulated or not for 6 hours and luciferase activity was measured and normalized (left panels). Values represent the mean plus or minus the standard deviation from triplicate samples.

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