Figure 1.
Biosynthesis of LTB4 by B-CLL cells. Intact B-CLL cells (10 × 106) were incubated for 5 minutes at 37°C with calcium ionophore A23187 (1 μM), arachidonic acid (AA; 40 μM) or A23187 (1 μM) plus AA (40 μM). The cells were preincubated for 2 minutes at 37°C, in the presence or absence of diamide (100 μM), prior to addition of indicated compound(s). Sonicated cells were preincubated with ATP (1 mM) for 2 minutes at 37°C and then incubated with calcium chloride (2 mM) and AA (40 μM) for 5 minutes. The results show the mean ± SD from 6 B-CLL patients.
