Figure 2.
![Figure 2. Induction of T-cell hyporesponsiveness by immature DCs. Peripheral blood CD4+CD45RO– T cells were stimulated with immature or mature allogeneic DCs 1, 2, or 3 times. (A) At the end of each stimulation period, T cells were tested for their ability to proliferate in response to mature allogeneic DCs. (B) In addition, after the third round of activation, their proliferative response to polyclonal activation was tested by stimulation with immobilized anti-CD3 mAb (1 μg/mL), in the absence or presence of soluble anti-CD28 mAb (1 μg/mL) and IL-2 (100 U/mL). After 48 hours of culture, [3H]-thymidine was added for an additional 16 hours. Results are representative of 17 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/3/10.1182_blood-2004-03-1211/6/zh80030573190002.jpeg?Expires=1735557834&Signature=mPisYpDEdeuHbCoLdp81K1LWeL1wFyb~Ek~fzRd0Y8uoFrqgeutsO1uYIyOIgTZvFdRJ4xJvW24fhPx0HdK7-m63PBHgHTd8J8Cjct6xjDZgj1eqblgIA~NIX3nYzaH0g1uBfi2I8pBCyEW2Pu5DZqqbQXxe4ytsFguiz~o9PTVojVO2b6fzo8qfIYsg~eDkG-GWlVF-KPEdwlS9aeFJ3o5C0RLpjPEiGmQ7W61GJ~hRoZSF4J7s8kAlFUA0BKkbvjrj5O5xV2x5HTUCDXl-NFdoG7ZDi8jIdqtykCwKBwzFV3-XALwBM9J4zwA2ZNZ5Dtr6VYCzSU67~9~IkUGSdw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Induction of T-cell hyporesponsiveness by immature DCs. Peripheral blood CD4+CD45RO– T cells were stimulated with immature or mature allogeneic DCs 1, 2, or 3 times. (A) At the end of each stimulation period, T cells were tested for their ability to proliferate in response to mature allogeneic DCs. (B) In addition, after the third round of activation, their proliferative response to polyclonal activation was tested by stimulation with immobilized anti-CD3 mAb (1 μg/mL), in the absence or presence of soluble anti-CD28 mAb (1 μg/mL) and IL-2 (100 U/mL). After 48 hours of culture, [3H]-thymidine was added for an additional 16 hours. Results are representative of 17 independent experiments.
