Figure 3.
![Figure 3. Real-time RT-PCR analysis of gene expression repressed in response to hypoxia or AdCA5. Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]), the fold change in the levels of CCNE1, MPHOSP9, POLD, POLG, CDC2, PRC1, RRM2, and NEK4 mRNA was calculated. The mean and standard error for the 3 independent data sets are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/2/10.1182_blood-2004-07-2958/6/zh80020572780003.jpeg?Expires=1735502078&Signature=KlzMmbSQsExBnE2~ZiJc7HBsosO67ZK1IEbSwptccpInJRohWL99~yeMUPiQfncu-tlw5zxjuE864yM~pWof5yVmLvV9KjRooyZF9yXyi1eOcKZ5Q-gmXGfP47wCx36e5uct326lGVY62DcqZVb2w59OQDGJtIR1rjpeXqtR9R1vrGjPqxVKNo9zK2HoaEC6quQgYReLLC2Hk8X7oqSXhDDRWK1pxgUx4Sk~cjtB15sjCXmoHPeRj-ZEGC7JRla9ykZC7uc5xO3qj71isZj8tByRLvGAiBvGSvlCcuozT78Z7VIZjRl4hKT6Tfbn0Pq0Yqgzkp4LOTxZloT5gR0sGg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Real-time RT-PCR analysis of gene expression repressed in response to hypoxia or AdCA5. Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time RT-PCR. For each pair of experimental conditions (ie, 1% O2 vs 20% O2 [H] and AdCA5 vs AdLacZ [C]), the fold change in the levels of CCNE1, MPHOSP9, POLD, POLG, CDC2, PRC1, RRM2, and NEK4 mRNA was calculated. The mean and standard error for the 3 independent data sets are shown.
