Figure 4.
Kinexus apoptosis screen shows multiple changes in protein levels. Western blots of mock-transfected (A) and Meis1b-transfected (B) protein lysates. Each lane was probed with up to 3 different specific primary antibodies followed by incubation with the appropriate secondary antibody and was visualized using enhanced luminescence. (C) Protein expression levels from lysates derived from mock-transfected and murine Meis1b-transfected cells were analyzed from SDS-PAGE gels probed with multiple apoptosis protein-specific antibodies. Band intensities were measured for each protein. The analysis algorithm used is based on the assumption that equal levels of loaded protein will have equal intensities. In generating the final report, the algorithm determines the total counts on each blot and corrects each band's count such that the resultant total counts on the blots equal the total average counts among all blots. Levels of mock-transfected samples were arbitrarily set to 0 for each protein and were compared with Meis1b-transfected samples. Graph shows the percentage difference from the mock sample. Transfection efficiency, 36%.