Figure 1.
![Figure 1. Fluorescent-tagged versions of FA proteins are functional. Mutant FA-G (EUFA143), FA-A (HSC72), and FA-C (HSC536N) cell lines were transfected with fluorescent-tagged wild-type ECFP-FANCG, EGFP-FANCA, and EYFP-FANCC cDNAs and the nonfluorescent tagged versions, respectively. The cells were treated with the indicated doses of MMC for 5 days and analyzed by XTT (2,3-Bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) assay. Each tagged version restored MMC resistance to the level of the nontagged version.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/2/10.1182_blood-2004-01-0001/6/zh80020572340001.jpeg?Expires=1735552122&Signature=blpqen3nS3iNaJFsEr9sGPzbNeVH798mcRCiS0Lnhx6maY0hXTT76wLPV4JqGXx0S3XeheTFRrrZmisnuEC1Uy3ckXa7oTH2LW~q~dLkTQYFZm3adRPXQHYBn-TudD26mRRGnt7klRYiclbttBh6ZfKZXn7ytJjiP2wSCf9ePChWr~HZ3UUKJTRnSX3W8Qyz9zr3vTppJeQEdxHucSyALZyn4rAavMG9u35D9BQ~7XMPxnrsTrDR8W3E7y6hQLGZZDk6bLx5CCZPL9UgzJ6-yMN3Q0GmQqFJr0ujTHzz0YdyPdqEi0-L6Cf2KxL~VhS0mc9ZQ0sHGSR8GI4NEnJ01w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fluorescent-tagged versions of FA proteins are functional. Mutant FA-G (EUFA143), FA-A (HSC72), and FA-C (HSC536N) cell lines were transfected with fluorescent-tagged wild-type ECFP-FANCG, EGFP-FANCA, and EYFP-FANCC cDNAs and the nonfluorescent tagged versions, respectively. The cells were treated with the indicated doses of MMC for 5 days and analyzed by XTT (2,3-Bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) assay. Each tagged version restored MMC resistance to the level of the nontagged version.
