FANCA, FANCC, and FANCG colocalize in chromatin. (A) HeLa cells were transfected with either GFP-FANCA/CFP-FANCG or CFP-FANCG/YFP-FANCC. Whole cells were fixed or chromatin preparations were performed in situ. (B) HeLa cells were transfected with EGFP, EYFP, or ECFP only–containing plasmid, and whole cells and chromatin were prepared. No GFP, YFP, or CFP signal was discernible on chromatin after preparation. Also, CFP was not visible through GFP filters, and YFP and GFP were not visible through CFP filters. (C) Point mutant versions of EGFP-FANCA(H1110P), EYFP-FANCC(L554P), and ECFP-FANCG(G546R) were transfected into HeLa cells. Whole cells contained none of the mutant proteins in the nucleus, and no mutant proteins were visible at all after chromatin preparation.