Figure 4.
FA core complex localizes to chromatin fibers. HeLa cells transfected with EGFP-FANCA/CFP-FANCG were synchronized and collected as in Figure 3. The mitotic cells were cytospun onto a new slide. Chromatin fibers were then prepared on slides by vertical urea extraction. Micrographs show foci, then diffusion of FA proteins on fibers through S phase, clumping in G2, and absence by mitosis.
