Figure 2.
Figure 2. Western blot analysis of expression of Stat6 or Stat4 proteins in cultured MMCs. Indicated cell lysates were blotted with anti-Stat6 (M-20), anti-Stat4 (C-20), and anti–α-tubulin Abs. (A) Western blot analysis of cultured MMCs derived from control, MITF-KD, Stat6-deficient, and Stat4-deficient mice. (B) Western blot analysis of cultured MITF-KD MMCs after enforced expression of control vector, MITF, or Stat6VT (activated form Stat6). Lysates of the indicated cultured MMCs were blotted with anti-Stat6, and anti-Stat4 and anti–α-tubulin Abs. (C) Western blot analysis of cultured Stat6-deficient MMCs after the enforced expression of control vector, MITF, or Stat 6VT. Lysates of the indicated cultured MMCs were blotted with anti-Stat6, and anti-Stat4 and anti–α-tubulin Abs. (D) Western blot analysis of expression of Stat4 proteins in cultured CTMCs. Cell lysates were blotted with anti-Stat 4 and anti–α-tubulin Abs.

Western blot analysis of expression of Stat6 or Stat4 proteins in cultured MMCs. Indicated cell lysates were blotted with anti-Stat6 (M-20), anti-Stat4 (C-20), and anti–α-tubulin Abs. (A) Western blot analysis of cultured MMCs derived from control, MITF-KD, Stat6-deficient, and Stat4-deficient mice. (B) Western blot analysis of cultured MITF-KD MMCs after enforced expression of control vector, MITF, or Stat6VT (activated form Stat6). Lysates of the indicated cultured MMCs were blotted with anti-Stat6, and anti-Stat4 and anti–α-tubulin Abs. (C) Western blot analysis of cultured Stat6-deficient MMCs after the enforced expression of control vector, MITF, or Stat 6VT. Lysates of the indicated cultured MMCs were blotted with anti-Stat6, and anti-Stat4 and anti–α-tubulin Abs. (D) Western blot analysis of expression of Stat4 proteins in cultured CTMCs. Cell lysates were blotted with anti-Stat 4 and anti–α-tubulin Abs.

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