Th1-related molecules in mast cells. (A) Western blot analysis of expression of IL-18Rα and NOS2 proteins in cultured MMCs derived from control, MITF-KD, and Stat6-deficient mice or cultured CTMCs derived from control mice. (B) IFN-γ production by cultured MMCs and cultured CTMCs. Culture supernatants of the indicated cultured mast cells with stimulation of IL-12 and IL-18 were analyzed with ELISA.