hCNT3 expression. (A) Scatter plot of hCNT3 expression. The distribution of hCNT3 expression among 45 samples of CLL is demonstrated in this graph. The expression of hCNT3 related to reference RNA levels is expressed in arbitrary PCR units. (B) Maximized significance of difference: hCNT3 expression dichotomization. In order to determine the optimal cut point value to maximize the significance of the difference between groups, the hCNT3 dataset was analyzed for each of 20 cut point groups (g1-g20), Kaplan-Meier curves were constructed, and log-rank analysis was performed. The scale on the left axis represents the log-rank–derived relative risk (), whereas the scale on the right represents statistical significance (P value; ⋄). The curves separated maximally in groups 7, 8, and 9, corresponding to a hCNT3 expression value of 0.4 (arbitrary PCR units, see Figure 4).