Trolox potentiates As2O3-mediated oxidative stress. (A) NB4, IM9, and AsR2 cells were treated with As2O3 and trolox for 24 hours. Western blot was used to determine HO-1 protein levels. β-Actin was used as a loading control. These data represent 3 independent experiments. (B) Protein carbonyl content was detected by ELISA in NB4 cells treated with As2O3 alone, trolox, or the combination for 3 days with the concentrations indicated. Data depicted are representative of 3 independent experiments each performed in duplicate. Asterisks indicate significant differences from As2O3-treated cells. (*P < .05; ***P < .001). (C) 8-iso PGF2α was detected in whole cells extracts from NB4 cells treated with the indicated compounds for 3 days. Standard deviation bars are shown. Asterisks indicate significant differences from As2O3-treated cells (P < .001).
