Multimeric structure analysis of VWF. VWF multimeric structure was analyzed by 2% SDS–2% agarose electrophoresis (see “Materials and methods”). Lane 1 indicates normal plasma; lane 2, purified VWF; lane 3, VWF/FVIII concentrate (supplied by Kaketsuken, Kumamoto, Japan). After adjusting the concentration of the samples to 1 VWF:RCo U/mL, they were subjected to electrophoresis. These VWF samples all contained VWF with similar multimeric structure.