LPC induces J774A.1 cell chemotaxis but not chemokinesis. (A) An LPC gradient is required for J774A.1 cell migration. The migration of G2A^HIGH J774A.1 cells was tested in transwell assay. LPC-induced J774A.1 cell migration was abolished by adding the same concentration of LPC into the upper chamber of the transwell. LPC alone in the upper chamber did not stimulate J774A.1 cell migration. The results are representative of 3 independent experiments. (B) Zigmond chamber assay. Morphological orientation of G2A^HIGH J774A.1 cells toward the chemoattractant LPC was examined in a Zigmond chamber. Migrating cells with extending lamellipodia and retracting tails were observed.