G-protein signaling in LPC/G2A-mediated chemotaxis. (A) Chemotaxis of J774A.1 cells overexpressing wild-type (G2A^HIGH) or DRY mutant G2A (G2A^ARY). J774A.1 cells were transduced with wild-type or DRY mutant (DRY to ARY) G2A to overexpress these proteins. Effects of the mutation were assessed by examining cell migration toward LPC. The results are representative of 4 independent experiments. (B) Western blot of G2A proteins. Wild-type and DRY mutant G2A proteins were detected at similar level with G2A antibody.