G_i and G_s signaling in LPC/G2A-mediated chemotaxis. (A, B) Effects of pertussis toxin on chemotaxis of wild-type and G2A^HIGH J774A.1 cells to LPC and C5a. J774A.1 cells were pretreated with 100 ng/mL PTX for 16 hours. 20 μM LPC was used as a chemoattractant to induce migration of PTX-treated or untreated J774 cells. Chemotaxis toward C5a was used as a control that is sensitive to PTX. The results are representative of 9 independent experiments. (C) G_s signaling in LPC/G2A-mediated chemotaxis. G2A^HIGH J774 cells were pretreated with 100 μM of2′,5′-dideoxyadenosine (DDA) for 1 hour to specifically inhibit adenylate cyclase or with the same volume of DMSO as a control. 20 μM LPC was used as a chemoattractant to induce migration of DDA-treated or untreated J774 cells. The results are representative of 3 independent experiments.