G_q/11 and G_12/13 signaling in LPC/G2A-mediated chemotaxis. (A) Effects of Gα dominant-negative mutants on J774A.1 cell migration to LPC. G2A^HIGH J774A.1 cells were transduced with dominant negative Gα subunits, Gα_q Q209L/D277N, Gα₁₁ Q209L/D277N, Gα₁₂ Q231L/D299N, and Gα₁₃ Q226L/D294N to specifically inhibit G_q/11 and G_12/13 signaling. Migration of these cells to 20 μM LPC was compared. The results are representative of 3 independent experiments. (B) Effects of Gα dominant negative mutants on J774A.1 cell migration to C5a. (C) Inhibition of J774A.1 cell migration to LPC by RGS constructs. G2A^HIGH J774A.1 cells were infected with GRK2 RGS or p115 RGS, and chemotaxis to LPC was examined. The results are representative of 3 independent experiments. (D) Effects of RGS constructs on C5a induced J774A.1 cell migration.