Validation of microarray data by real-time RT-PCR, Western blotting, and flow cytometry analysis. (A) Total RNA was purified from BM and PB populations and subjected to real-time RT-PCR and microarray analysis to compare gene expression profiles for selected marker genes of granulocytic differentiation (mean ± SD, n = 3). (B) Comparison of protein and mRNA profiles in BM and PB populations assessed by Western blotting, flow cytometry, and microarray analysis (mean ± SD, n = 3).