American Society of Hematology

Figure 3.

$Figure 3. HOXB6-immortalized cell lines are able to differentiate into neutrophils and monocytes when grown in the presence of G-CSF or M-CSF, respectively. (A) Representative photomicrographs from HOXB6 and HOXB6WG cell lines grown in the presence of GM-CSF, G-CSF, or M-CSF. Images were acquired as described for Figure 1. (B) Morphologic evidence of differentiation is accompanied by characteristic changes in gene transcription measured by real-time Q RT-PCR. Granulocytic differentiation is accompanied by up-regulation of MMP9 and gp91pHOX, whereas expression of MSR2, a gene normally expressed late in monocytic differentiation, is down-regulated. M-CSF–induced monocytic differentiation, in contrast, is accompanied by up-regulation of MSR2 in addition to less pronounced increases in MMP9 and gp91pHOX. Results shown are for 2 independently derived HOXB6 cell lines. Q RT-PCR assays were performed in triplicate. (C) Differentiation in response to G-CSF or M-CSF occurs without significant change in HOXB6 expression as assayed by Western blot. Cells grown in GM-CSF containing media and whole unfractionated bone marrow were used as positive and negative controls, respectively.$

HOXB6-immortalized cell lines are able to differentiate into neutrophils and monocytes when grown in the presence of G-CSF or M-CSF, respectively. (A) Representative photomicrographs from HOXB6 and HOXB6WG cell lines grown in the presence of GM-CSF, G-CSF, or M-CSF. Images were acquired as described for Figure 1. (B) Morphologic evidence of differentiation is accompanied by characteristic changes in gene transcription measured by real-time Q RT-PCR. Granulocytic differentiation is accompanied by up-regulation of MMP9 and gp91pHOX, whereas expression of MSR2, a gene normally expressed late in monocytic differentiation, is down-regulated. M-CSF–induced monocytic differentiation, in contrast, is accompanied by up-regulation of MSR2 in addition to less pronounced increases in MMP9 and gp91pHOX. Results shown are for 2 independently derived HOXB6 cell lines. Q RT-PCR assays were performed in triplicate. (C) Differentiation in response to G-CSF or M-CSF occurs without significant change in HOXB6 expression as assayed by Western blot. Cells grown in GM-CSF containing media and whole unfractionated bone marrow were used as positive and negative controls, respectively.

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