Figure 6.
Contribution of PAF and β2 integrin on angiopoietin-induced neutrophil adhesion onto HUVECs. Neutrophils were pretreated with increasing concentrations of CV-3988 (10–8 to 10–6 M; A) and blocking anti-CD18 IgG (1 to 5 μg/mL; B) for 15 minutes prior to the adhesion assay. Neutrophils were added to HUVECs concomitantly to their stimulation with Ang1, Ang2 alone, or the Ang1/Ang2 combination (10–9 M; 7.5 minutes). Data are means ± SEM of at least 8 experiments; *P = .01 compared with PBS, §P = .01 compared with corresponding agonist.
