Tumor xenografts sections and peripheral blood smears. Hematoxylin and eosin low-power images of hepatoma xenografts expressing GFP (A) or hepcidin and GFP (B) show sheets of neoplastic hepatocytes, abnormal blood vessels, and areas of hemorrhage. Corresponding Perl Prussian blue-stained sections (C-D) demonstrate hemosiderin-laden macrophages in the fibrous capsule surrounding the tumors. Scale bars represent 50 μm. Panels E and F show Giemsa-stained blood smears from a pair of similarly severely anemic (Hgb level 68 vs 67 g/L) mice with hepatoma xenografts either not engineered or engineered to express hepcidin, respectively, on a high-iron diet. Arrows point to some of the many erythrocyte fragments.