Effects of RhoH overexpression on hematopoietic progenitor cell engraftment in the lethally irradiated recipient mice. LDBM cells were transduced with MIEG3-HA-RhoH and vector control. A mixture of 40% transduced (EGFP⁺) and 60% untransduced (EGFP^–) competitor cells was transplanted into the lethally irradiated recipient mice. (A) Expression of HA-RhoH protein in BM cells of the recipient mice at 6 months after transplantation. The nucleated BM cells from the representative recipients were used for immunoblot analysis probed for HA tag or β-actin as a loading control. The result is a representative of 3 experiments. (B) Reduced engraftment of RhoH-transduced HSC/Ps. The engraftment in PB of each recipient mouse was determined monthly by flow analysis of EGFP⁺ chimerism. Data represent the mean ± SD; n > 10 mice per group. *P < .01, RhoH-versus vector-transduced cells. (C) Engraftment and lineage distribution of RhoH-transduced cells in PB, BM, spleen, and lymph node of the recipients at 6 months after transplantation. Data represent the mean; n > 10 mice per group. *P < .01, RhoH-versus vector-transduced cells.