Antiproliferative effect of BIBR1532 in JVM13 leukemia cell line. (A) JVM13 cells were cultured for 9 days in RPMI medium supplemented with increasing concentrations of BIBR1532. Cells were counted at indicated time points using trypan blue exclusion assay. (B) The viability of JVM13 cells treated with 80 μM was determined at indicated time points using forward scatter (FSC)/side scatter (SSC) pattern of FACS analysis. A gradual decrease in viability was observed with cells cultured in the presence of 80 μM BIBR1532. Values represent means and SEM from triplicates. (C) Proliferation of JVM13 cells was determined using WST-1 assay.36 Bars represent the proliferation of cells cultured in the presence of 80 μM BIBR1532 as percentage of controls at indicated time points. Values are means and SEM from triplicates.
