Western blot analysis of TRF2 and p53/phospho-p53 in leukemia cells treated with BIBR1532. Protein was extracted using RIBA buffer from HL-60 and JVM13 treated with 80 μM BIBR1532 at indicated time points. Western blot analysis was performed using a monoclonal (A) anti-p53 and anti-phosphorylated p53 (dilution 1:1000) and (B) anti-TRF2 antibody (dilution 1:250). Actin expression levels were used as internal control.