Long-term regulated expression of Epo using first-generation AAV vectors. Primate 94B091 was cotransduced with AAV-CMV-TF1 and AAV-Z12I-rhEpo-2 once at the beginning of the study (Table 1 shows the doses), and subsequently administered rapamycin (▵) or on one occasion rapamycin analog AP1861 (⋄) intravenously at the indicated doses (mg/kg). Epo levels (•) peaked at 495 mU/mL after the first induction. Hematocrit levels (⋄ with solid line) are also given.