Long-term regulated expression of Epo using a single AAV vector. (A) AAV2 single vector. Primate 99E120 was transduced with AAV-TF-rhEpo-2.3 once on day 1, and subsequently administered AP22594 (▾) intravenously at the indicated doses (mg/kg). Serum Epo levels (• with solid line) and hematocrit levels (⋄ with solid line) are also given. (B) AAV1-pseudotyped vector. Primate 00E022 was transduced with an AAV1-pseudotyped AAV-TF-rhEpo-2.3 vector once on day 1, at a 10-fold-lower dose than that administered to 99E120 (Table 1).