Figure 2.
WT retinal neovascularization. C57BL/6.gfp chimeric mice underwent the retinal ischemia model followed by perfusion with TRITC before eye enucleation and confocal imaging of the retinas. Panel A is the red channel alone. Panel B is the green channel alone. Panel C is the merged image of the red and green channels. Yellow areas are colocalization of donor-derived gfp cells and TRITC-perfused patent vessels. Note: All further panels in each figure are from red and green channel merged images unless noted. Panels D and F are contralateral untreated control eyes and E and G are experimental eyes from the same respective mice. Original magnification × 60; size bar about 10 μm.